Simultaneous determination of caffeine, theobromine and theophylline in foods and pharmaceutical preparations by using ion chromatography
نویسندگان
چکیده
Two ion chromatographic methods have been proposed for the simultaneous determination of caffeine, theobromine and theophylline. The separations were based on isocratic elutions and the determinations on ultraviolet absorbance detections at 274 nm. The detection limits (signal-to-noise ratio 3:1) for the three compounds were all below sub-mg mlÿ1 level. The methods have been successfully applied to the determination of these three compounds in foods and pharmaceutical preparations, and the average recoveries for various samples ranged from 87% to 103%. In addition, the retention mechanisms of these three compounds on various ion-exchange resins were discussed. The results obtained revealed that ion-exchange chromatography is possibly a good alternative to conventional liquid chromatography for the separation and determination of some water-soluble, neutral organic molecules with a certain hydrophobicity. # 1998 Elsevier Science B.V. All rights reserved.
منابع مشابه
Simultaneous HPLC determination of caffeine, theobromine, and theophylline in food, drinks, and herbal products.
A rapid and selective high-performance liquid chromatographic (HPLC) method is developed for the separation and determination of caffeine, theobromine, and theophylline. The chromatography is performed on a Zorbax Eclipse XDB-C8 column (4.6x150 mm i.d., 5-microm particle size) at 25 degrees C, with a mobile phase of water-THF (0.1% THF in water, pH 8)-acetonitrile (90:10, v/v). The flow rate is...
متن کاملSimultaneous determination of caffeine, theobromine, and theophylline by high-performance liquid chromatography.
This work relates the development of an analytical methodology to simultaneously determine three methylxanthines (caffeine, theobromine, and theophylline) in beverages and urine samples based on reversed-phase high-performance liquid chromatography. Separation is made with a Bondesil C18 column using methanol-water-acetic acid or ethanol-water-acetic acid (20:75:5, v/v/v) as the mobile phase at...
متن کاملHigh Throughput Separation of Xanthines by Rapid Resolution HPLC Application Note
Xanthines were found to be optimally separated by reversed-phase HPLC on a C18 column. By a reduction in column length and particle size, the separation time for a mixture of the xanthines investigated was reduced from 8 minutes to 1.5 minutes without a major loss in resolution. A simple isocratic HPLC method was used and High Throughput Separation of Xanthines by Rapid Resolution HPLC Applicat...
متن کاملUV-Vis Spectrophotometry and Multivariate Calibration Method for Simultaneous Determination of Theophylline, Montelukast and Loratadine in Tablet Preparations and Spiked Human Plasma
Resolution of binary mixtures of theophylline (THEO), montelukast (MKST) and loratadine (LORA) with minimum sample pretreatment and without analyte separation has been successfully achieved by multivariate spectrophotometric calibration, together with partial least-squares (PLS-1), principal component regression (PCR) and hybrid linear analysis (HLA). Data of analysis were obtained from UV–Vis ...
متن کاملاستفاده از سلول های در حال استراحت سویه ی بومی غربال گری شده Rhodotorula sp. CW03 در بیوترانسفورماسیون کافئین به تئوفیلین و پاراگزانتین
Introduction & Objective: In recent years, microorganisms have been applied as biocatalysts for making pharmaceutically natural products. Microbial biotransformation of caffeine suggests a dual approach for biodegradation of toxic caffeine from polluted environments and a method for the production of medically and pharmaceutically valuable dimethylxanthines. The present work describes the ident...
متن کامل